Effect of superparamagnetic iron oxide nanoparticles (SPIONs) on thyroid cells.  

PERONA M; GRISSI C; THOMASZ L; OGLIO R; RODRIGUEZ C; ROSEMBLIT C; CAMPOS HAEDO M; DAGROSA MA; CREMASCHI G; DURAN H; JUVENAL G; IBAÑEZ I

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Sociedad argentina de investigación clínica

Introduction: Thyroid carcinoma is the most frequent malignancy of the endocrine system. Well-differentiated thyroid carcinoma, especially papillary (PTC) and follicular (FTC) variants, account for about 94% of the cases and the prognosis is favorable. However, there remains a subset of patients with advanced or recurrent disease with a poorer response to conventional therapy. External beam radiotherapy (EBRT) is a treatment option especially for papillary thyroid carcinoma. Superparamagnetic iron oxide nanoparticles (SPIONs) have been used in cancer diagnosis and therapy. SPIONs or mPEG-SPIONs increase sub-toxically ROS levels. This characteristic could be combined with radiotherapy to optimize the clinical outcome. Objective: The aim was to study the effect of SPIONs in different thyroid cell lines. Methods: SPIONs were synthesized and stabilized by methyl-poly(ethylene glycol). Papillary thyroid cancer cells (TPC-1) were incubated with different concentrations of coated (mPEG) and non-coated SPIONs. Cell viability was measured 24 and 48 hours later by MTT method. Intracellular SPION content by measuring the Fe concentration per cell was performed by ICP-AES at 2, 4 and 24 hours. Intracelullar ROS levels were measured using the fluorescent dye 2´, 7´-dichlorofluorescein-diacetate (DCFH-DA). Results: Increasing doses of SPIONs did not affect cell viability (0-250 µg/ml). Intracellular iron content per cell was significantly increased at 2, 4 and 24 hours in cells incubated with SPIONs (p