EXPERIMENTAL STUDIES FOR THE PERSONALIZED APPLICATION OF BORON NEUTRON CAPTURE THERAPY (BNCT) TO THE TREATMENT OF CUTANEOUS MELANOMA (CM)

CARPANO M,; SANTA CRUZ G,; RODRIGUEZ C; NIEVAS S; OLIVERA M.; PERONA M; BOGGIO E; LONGHINO J,; PISAREV M; JUVENAL G; DAGROSA MA

Mar del Plata

Congreso; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC)

Boron Neutron Capture Therapy (BNCT) is a binary modality based on the nuclear reaction 10B (n, α) 7Li that has been used to treat a variety of tumors between these, cutaneous melanoma (CM). In previous studies in agreement with the personalized oncology we have demonstrated that boronophenylalanine (BPA) uptake it can be correlated with the tumoral temperature and viability. Thermal conductivity is associated with the material; in biological tissues this can include the protein, fat and water content of each tissue. With thermography you can see the outside, the surface. To derive what happens inside we use a model that uses the heat transport equation or Fourier equation.Purpose: develop a heat transport model that allows knowing the thermal conductivity of the tumor tissue, this physical property allows us to relate the thermal conductivity with the relationship in the concentration of boron between tumor and blood, to predict tumor response in BNCT therapy from the thermal conductivity of tumor tissue. Methods and Materials: Nude mice implanted with human melanoma cells Mel J were divided into different groups (Control, NCT, BNCT) and irradiated with the thermal neutron beam from RA 6 (4.96 x108 n/cm2sec). Tumor and body temperatures were measured by Static Infrared Imaging (SIRI), and the follow up of the animals was performed. A heat transport model was developed, considering conduction, convection and with an internal heat source. From this model, the thermal conductivity of the tumor is derived based on the internal and external temperatures, plus the ambient temperature and the size of the tumor. Then the conductivity is correlated with the relationship in the concentration of boron between tumor and blood obtained from previous studies of biodistribution of the compound BPA. Infrared thermography studies and individualized tumor growth studies, allowing obtaining a curve that relates thermal conductivity and allows with the relationship in the boron concentration between tumor and blood (T / S).Results: Tumor growth showed a complete growth inhibition during the first 20 days after treatment in BNCT groups vs Control (p