Uso de Aphanocladium sp., Acremonium sp. o Acidithiobacilus ferrooxidans adsorbidos en una arcilla natural (montmorillonita), como sistema para retener Uranio

MELISA OLIVELLI, GUSTAVO CURUTCHET Y ROSA TORRES.

Carlos Paz

Congreso; VI Congreso Argentino de Microbiología General (SAMIGE). Carlos Paz, Cordoba. Octubre 2009; 2009

SAMIGE

Uranium is a radionuclide with noticeable toxicity as heavy metal. It reaches water from natural and man-made sources representing a risk for human health and environment. Generally, conventional sorption techniques for heavy metals removal from wastewaters are not useful due to the great volumes treated and the low concentration of pollutants. Biosorption is an alternative process where different types of biomass allow heavy metal concentration from diluted solutions. Among the most commonly used biosorbents is the fungal biomass, with the advantage of being easily generated at low costs. The main technological drawback in biosorption processes is getting a suitable immobilization of the biomass to obtain efficient biofilters. A methodology to increase biosorption surface and retain biomass is to generate clay biopolymers matrices. Montmorillonite clays are among the possible innocuous sorbents with this characteristic. This type of clay has optimal properties for metal sorption because it is able to complex all kind of organic as well as inorganic compounds on its surface. In this study, we use microorganisms able to grow in the presence of high concentrations of Uranium: Aphanocladium sp., Acremonium sp., and the acidophilic bacteria Acidithiobacillus ferrooxidans. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations. acidophilic bacteria Acidithiobacillus ferrooxidans. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations. Aphanocladium sp., Acremonium sp., and the acidophilic bacteria Acidithiobacillus ferrooxidans. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations. Acidithiobacillus ferrooxidans. U(VI) adsorption by microbial-natural clay matrices was studied to determine if biomass immobilization on clay could increase the montmorillonites sorption capacity. The different generated matrices were characterized by X- ray power diffraction (XRD), specific surface area and scanning electron microscopy. Uranium sorption capacity was determined by batch systems. Analyzed clays exhibited a great capacity for uranium sorption. This property was remarkably modified by interaction with different microorganisms and culture media. Fungal biofilms that grew on clay with an organic carbon source in the culture medium increased sorption capacity. Furthermore, the separation process was easier as the presence of the biofilm facilitated clay precipitation. The simple sorption of fungal and bacterial biomass on clay matrices also produced an increase in the Uranium sorption capacity. These preliminary studies conclude that these microbial biofilms-clay systems have a great potentiality for uranium biosorption processes given its high cationic exchange capacity and its ability to adapt the process to different situations.