Estrogen receptor is involved in the impairment of bile salt secretion induced by estradiol-17beta-glucuronide in isolated rat hepatocyte couplets

BAROSSO, ISMAEL; ZUCCHETTI, ANDRÉS ERNESTO; TABORDA, DIEGO; CROCENZI, FERNANDO A.; SÁNCHEZ POZZI, ENRIQUE J

Copenhague, DINAMARCA

Congreso; 44th Annual Meeting of the European Association for the Study of the Liver; 2009

European Association for the Study of the Liver (EASL)

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Recently, estrogen receptors (ER) have been implicated in the chronic estrogen cholestasis model induced by ethynylestradiol (JBC 2006, 281:16625−31). The aim of this study was to evaluate the involvement of ER in E17G-induced changes in bile salt canalicular excretion. Methods: Isolated rat hepatocytes couplets were cultured for 5 h, exposed to the estrogen receptor inhibitor ICI-182,780 (ICI, 1 mM) for 15 min and then incubated with E17G (50 mM) for 20 min. Other couplet preparations were additionally incubated with PKC inhibitor Go6976 (Go, 1 mM, 15 min) before E17G incorporation. Finally, all preparations were incubated with cholyl-lysylfluorescein (CLF) a fluorescent bile salt substrate of Bsep. Couplets accumulating CLF in their vacuole were counted in a fluorescent microscope and informed as a percentage. Results were compared by ANOVA followed by Student-Newman-Keuls test. Results: See the table. <!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} @font-face {font-family:"OGFDA H+ JS Times New"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"JS Times New"; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} @font-face {font-family:"OGFDD M+ CMS Y 10"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-alt:"CMS Y"; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} p.Default, li.Default, div.Default {mso-style-name:Default; mso-style-unhide:no; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; mso-layout-grid-align:none; text-autospace:none; font-size:12.0pt; font-family:"OGFDA H+ JS Times New","sans-serif"; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-bidi-font-family:"OGFDA H+ JS Times New"; color:black; mso-fareast-language:EN-US;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:10.0pt; line-height:115%;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} -->