Evaluation of the RhD protein and of membrane-bound IgG participation in the cord red blood cells senescence process.

MUFARREGE N; ENSINCK A; RACCA L; TRUCCO BOGGIONE C; LUJÁN BRAJOVICH M; MATTALONI S; GARCÍA BORRÁS S; BIONDI C; COTORRUELO C

Rosario

Congreso; XVIII Congreso y XXXVI Reunión Anual de la Sociedad de Biología de Rosario; 2016

Sociedad de Biología de Rosario

While neonates red blood cells (RBCs) survive in circulation between 60-80 days, adults RBCs survive approximately 120 days. Multiple alterations trigger the selective removal of the Senescent (Se) RBCs from the circulation. In adults, erythrocyte aging may l ead to autologous IgG binding followed by recognition and phagocytosis. However, the senescence process in neonatal RBCs is not fully characterized. We evaluated the participation of the RhD protein in the erythrocyte aging and we analyzed membrane bound IgG in Se and Young (Y ) RBCs populations obtained from cord blood samples using flow cytometry. For the RhD protein study, samples (n=18) were incubated with APC anti- RhD IgM antibodies. Median fluorescence intensity (MFI) was measured. To assess the membrane bound IgG, samples (n=15) were incubated with an Alexa 488 anti-human IgG antibody. The percentage of opsonized RBCs with IgG was measured in both populations. The mean of MFI in cord YRBCs was significantly higher when compared with SeRBCs (Y=878.9±231.33 vs Se=756.1±166.95, p