Lipase of Candida albicans induces activation of NADPH oxidase and L-arginine pathways on resting and activated macrophages.

PARAJE MG, CORREA SG, ALBESA I, SOTOMAYOR CE.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2009 vol. 390 p. 263 - 268

0006-291X

Candida albicans secretes various hydrolytic enzymes which are considered to be an integral part in the 22albicans secretes various hydrolytic enzymes which are considered to be an integral part in the 22 pathogenesis. However, the role of lipases is far from being completely understood and the direct effects 23However, the role of lipases is far from being completely understood and the direct effects 23 of these fungal enzymes during the host–pathogen interaction remain to be established. We recently iso- 24host–pathogen interaction remain to be established. We recently iso- 24 lated and characterized an extracellular C. albicans lipase (CaLIP), and demonstrated the ability of this 25C. albicans lipase (CaLIP), and demonstrated the ability of this 25 fungal enzyme to interact directly with macrophages (M/) and hepatocytes and to operate as a virulence 26/) and hepatocytes and to operate as a virulence 26 factor. Herein, we explored the effects of CaLIP on M/ functions such as oxidative burst and L-arginine 27Herein, we explored the effects of CaLIP on M/ functions such as oxidative burst and L-arginine 27 metabolism. The study was performed in cells with different activation status: normal-resting M/s 28/s 28 and M/s primed in vivo or in vitro with C. albicans. The ability of this fungal factor to modulate the 29/s primed in vivo or in vitro with C. albicans. The ability of this fungal factor to modulate the 29 above-mentioned parameters was dependent on cells status, dose, and microenvironment, where the 30parameters was dependent on cells status, dose, and microenvironment, where the 30 interaction took place. These results constitute a new finding in the biology of candidiasis and could illus- 3131 trate an additional evolutive advantage for the fungus in the framework of the bidirectional host–path- 32host–path- 32 ogen interaction.interaction.