INVESTIGADORES
CERVI Laura Alejandra
congresos y reuniones científicas
Título:
Cathepsin L3 from Fasciola hepatica induces IL-6 and IL-10 production in murine dendritic cells through the toll like receptor
Autor/es:
CELIAS D; CORVO I; TORT J; SILVANE L; MOTRAN CC; . CERVI L.
Lugar:
Mar del Plata
Reunión:
Congreso; LXII Reunión anual. Sociedad Argentina de Inmunología; 2014
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
(245) CATHEPSIN L3 FROM FASCIOLA HEPATICA INDUCES IL-6 AND IL-10 PRODUCTION IN MURINE DENDRITIC CELLS THROUGH THE TOLL-LIKE RECEPTOR
4 SIGNALING
Celias, Daiana Pamela1; Corvo, Ileana2; Tort, José2; Silvane, Leonardo1; Motrán, Cristina1; Cervi, Laura1
Facultad de Ciencias Químicas. Centro de Investigaciones
en Bioquímica Clínica e Inmunología (CIBICI) CONICETUNC1. Depto. de Genética, Facultad de Medicina. Universidad de la República, Uruguay2
During its migration through host tissue, the helminth parasite
F. hepatica, excrete-secrete a number of products among which
are cathepsins. These enzymes belong to the family of cysteineproteases that are essential for survival and colonization of the
parasite. While Cathepsin L1 is mainly secreted by adult parasites,
Cathepsin L3 (CL3) is released by juvenile worms. Recently, it
has been described a collagenolytic activity of this enzyme, which
may be relevant in the parasite?s ability to cross the intestinal wall,
thus continuing with its life cycle in the host. However, there is no
information about the interaction of this protein with immune cells
system. Playing dendritic cells (DC) a key role for sensing antigens
and inducing different adaptive responses, the aim of this work was
to investigate the ability of CL3 to modulate the murine DC maturation and its interaction with TLR receptors. Bone marrow-derived
DC from mice C57BL/6 mice were differentiated with GM-CSF
factor and cultured for 18 h with recombinant CL3, produced in
Hansenula polymorpha. After this time, CL3 didn?t show cytotoxic
activity on DC compared to untreated DC (Student?s test p =
NS), by cell viability assay with MTT 3 (4,5-di-methyl-thiazol-2-yl)
-2,5-di-phenyl tetrazoliumbromide. We also demonstrated that CL3
increases the levels of IL-6 and IL-10 (Student test, p