Epidermal host defense (antimicrobial) peptide gene expression in experimental dermatomycosis

BURSTEIN V; IGNACIO BECCACECE, LORENA GUASCONI, MARIEL ALMEIDA, CRISTIAN MENA; . CERVI L.; PISTORESI DE PALENCIA CRISTINA; CHIAPELLO L

Congreso; . LXIX Reunión Annual de la Sociedad Argentina de Inmunología (SAI); 2021

Host defense antimicrobial peptides (HDP) are small proteins thatdirectly kill or inhibit pathogen growth, modulate inflammation andskin homeostasis. Previously, we demonstrated that type 17 responseis crucial to inhibit fungal proliferation after dermatophyteinfection in C57BL/6 mice and, in the absence of a functional IL-17 pathway in IL-17RAKO mice, fungal burden was significantlyincreased compared to WT mice. However, the IL-17-mediated effectormechanisms that inhibit dermatophyte growth and the role ofHDP remain undefined.The aim of this work was to evaluate the gene expression of theHDP β-defensins 2, 3 and 14 and calprotectin (S100A9) in the epidermisof C57BL/6 and IL-17RAKO mice at early time-points of Nannizziagypsea infection.C57BL/6 (WT) and IL-17RAKO mice were epicutaneously infectedwith a N. gypsea mycelia suspension (infected group) or treated withsterile saline solution (uninfected controls). On day 1 or 3 post-infection(dpi), skin was trypsinized, mRNA was extracted, cDNA wasgenerated and used for quantitative PCR with primers for mBD2,mBD3, mBD14, S100A9, GAPDH and β-actin genes.Dermatophyte-infected WT mice expressed lower levels of mBD2compared to uninfected controls by 1 dpi (p