Solid-phase peptide synthesis using ChemMatrix, a based polyethylene glycol (PEG) solid support

FERNANDO ALBERICIO,; BAYO, N.; A CAMPERI, SILVIA; OSVALDO CASCONE; SIMON COTE,; ERRACHID, A.; FURIC, R.; MARTÍN, F. G.; IANNUCCI, NANCY B; M. MARANI, MARIELA

San Diego

Congreso; 19th American Peptide Symposium; 2005

    SOLID-PHASE PEPTIDE SYNTHESIS USING  CHEMMATRIX, A BASED POLYETHYLENGLYCOL (PEG) SOLID SUPPORT F. Albericio,1 N. Bayo,1 S.A. Camperi,2 O. Cascone,2 S. Côté,3 L.J. Cruz,1 A. Errachid,1 R. Furic,3 F. Garcia-Martin,1 J. García-Ramos,1 N.B. Iannucci,2 M.M. Marani,2 M. Pla-Roca,1 J. Samitier,1 and J. Tulla-Puche.1 1University of Barcelona, Barcelona, Spain; 2UBA, Buenos Aires,  Argentina; 3Matrix Innovation Inc., Montreal, Quebec, Canada   Polystyrene (PS) and PEG-PS solid supports are presently the most used solid supports for the SPS.  Although, a large number of peptides and small molecules can be prepared using those supports, there is an increase need for the development of new ones for a more universal use.  In this sense, amphiphilic matrix contained just PEG are very attractive.  Herein, the new 100% PEG based resin, ChemMatrixR, is introduced.  ChemMatrixR, which is made exclusively from primary ether bonds and therefore highly chemically stable, has a good loading and is a user friendly resin because of its free flowing form once dried.   It shows an excellent performance for the preparation of hydrophobic peptides, such as T-20, (Ala)10-Val, as well as of one-bead-one-peptide libraries. NMR, SEM, Confocal Fluorescence Microscopy and nanotechnological techniques as AFM will be used to characterize ChemMatrix resin, study active groups distribution, and surface morphology bead by bead.           19th American Peptide Symposium San Diego, EEUU, 18 al 23 de junio de 2005