Organic compounds present in airborne particles stimulate superoxide production and DNA fragmentation: role of NOX and Xanthine Oxidase in animal tissues

TAVERA BUSSO, IVAN; SILVA GUILLERMO; CARRERAS, H.

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2016 vol. 23 p. 16653 - 16660

0944-1344

Suspended particulate matter (SPM) trigger the production of reactive oxygen species. However, most of these studies focused on the effects of specific compounds and not real mixtures. In order to study the enzymatic superoxide (O2-) production resulting from exposition to a complex mixture, we derived organic extracts (OE) from SPM containing polycyclic aromatic hydrocarbons (PAHs) and exposed to mammal tissues. DNA damage was quantified employing the comet assay. In all tissues, studied enzymes were involved in O2- production when they were exposed, as the lucigenin?s chemiluminescence decays when enzymes were inhibited. Same trend was observed with the percentage of cells with comets, as the DNA damage was higher when they were exposed to same conditions. Our data allows us to hypothesize that these enzymes play an important role in oxidative stress by PAHs and that there is a mechanism involving them in O2-generation.