Participation of human adipose tissue in the regulation of tumor progression in kidney cancer

BRUNA, F. A.; ROMEO, L.R.; CAMPO VERDE ARBOCCO, F.; CONTADOR, D.; LÓPEZ-LAUR,J. D.; GÓMEZ, S.E.; SANTIANO, F.E.; SASSO, C.V.; ZYLA, L.E.; LÓPEZ-FONTANA, C.M.; CARON, R.W.; PISTONE-CREYDT, V.

Merlo, San Luis

Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017

Sociedad de Biología de Cuyo

We have recently demonstrated that human adipose tissue of tumor kidney [hRAT], showed leptin and versican overexpressed compare to adipose tissue of normal kidney [hRAN]. Meanwhile, adiponectin and perilipin were significantly increased in hRAN. Conditioned media (CMs) of hRAN significantly increased proliferation of tumor [ACHN] and non-tumor [NK-2] renal epithelial cells. Now, we evaluated the expression of receptors (R): adiponectin -1, -2 (AdipoR1/R2) and leptin (ObR) in hRAN vs. hRAT and ACHN vs. HK-2. Additionally, CD44 expression (homing cell adhesion molecule) was assessed in cells. Human renal adipose tissues were obtained from patients with renal cell carcinoma [hRAT, n=6] and kidney donors [hRAN, n=8]. The CMs of hRAN and hRAT were collected 24h post incubation and cells were treated with CMs by 24 h. Protein expression was evaluated in tissue by IHQ, and WB in cells. Statistical differences among experimental conditions were evaluated by one-way ANOVA or t test, with Tukey´s post hoc tests. We found AdipoR1 increased and ObR decreased in hRAN vs. hRAT (p