Human renal adipose tissue from normal and tumoral kidney regulates the behavior of renal epithelial cells.

CAMPO VERDE ARBOCCO, F.; BRUNA, F. A.; ROMEO, L.R.; CONTADOR, D.; GÓMEZ, S.E.; SANTIANO, F.E.; SASSO, C.V.; ZYLA, L.E.; LÓPEZ-FONTANA, C.M.; CARON, R.W.; PISTONE-CREYDT, V.

Buenos Aires

Congreso; Reunión conjunta de sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica

Tumor stroma is one of the emerging topics of interestin cancer research. In the present work, we evaluated the effects ofConditioned Media (CMs) of human adipose tissue explants fromnormal (hRAN) and tumor (hRAT) kidney on proliferation of tumor(786-O, ACHN) and non tumor (NK-2) renal epithelial cells. We alsoevaluated: 1) leptin, adiponectin and versican mRNA expression inhRAN and hRAT; 2) adiponectin, ADAMTS1 and perilipin expressionin hRAN- and hRAT-CMs; and 3) adiponectin and perilipin expressionin hRAN- and hRAT.Human renal adipose tissues were obtained from patients with renal cell carcinoma (hRAT, n=6) and kidney donors (hRAN, n=8). TheCMs from hRAN and hRAT were collected after 24h of incubation.786-O, ACHN and NK-2 cells were grown and incubated with CMsfor 24 and 48h. Proliferation was quantified by MTT; mRNA expressionwas evaluated by real-time PCR, protein expression by WB andimmunohistochemistry. Statistical differences between experimentalconditions were evaluated by one-way ANOVA or t test. Tukey´spost hoc tests were performed.We found a higher RNA expression of leptin (a major adipokinewith tumorigenic effects) and versican (a proteoglycan that is essentialin epithelial-stromal interaction and intracellular signaling) inhRAT vs. hRAN explants (p