Identification of soluble components in human adipose tissue explants from renal cell carcinoma

CAMPO VERDE ARBOCCO, F.; LÓPEZ-LAUR,J. D.; ROMEO, L.R.; GIORLANDO, N.; LÓPEZ-FONTANA, G.; LÓPEZ-FONTANA, R.; SANTIANO, F.E.; SASSO, C.V.; ZYLA, L.E.; LÓPEZ-FONTANA, C.M.; CARON, R.W.; PISTONE-CREYDT, V.

Mendoza

Congreso; XXXIV Reunión Científica anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

We have recently demonstrated that conditioned media (CMs) from human adipose tissue explants from renal cell carcinoma near the tumor (hRATnT) regulate adhesion and migration of tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells, contrary to CMs from human adipose tissue explants from renal cell carcinoma farther away from the tumor (hRATfT). Now, we have begun to identify soluble components present: 1) in hRATnT- and hRATfT-CMs by WB; 2) in tumor (786-O, ACHN) and non tumor (NK-2) renal epithelial cells incubated with different CMs. We employed the CMs after 24 h of incubation of hRATnT and hRATfT. We evaluated the expression of proteoglycan versican, metalloproteinase ADAMTS1, leptin and adiponectin in the CMs. We incubated 786-O, ACHN and NK-2 cells with theCMs for 24 h, lisated the cells and evaluated the expression of AdipoR1, AdipoR2, ObR and leptin. We observed a higher expression of versican, leptin and adiponectin, as well as a lower expression of ADAMTS1 in hRATnT-CMs compared to the expression in hRATfT-CMs (p