PROPER BREAST FEEDING PROTECTS AGAINST MAMMARY CARCINOGENESIS IN ADULT LIFE

SANTIANO, F.; SASSO, C.V.; ZYLA, L.; PISTONE CREYDT, V.; SEMINO, S.; LÓPEZ-FONTANA, C.; CARÓN, R.W.

Mendoza

Congreso; XXXII Reunión Científica de la SBCuyo.; 2014

Sociedad de Biología de Cuyo

Cancer is one of the most important causes of morbidity and mortality worldwide, and in particular, breast cancer is the most common in women in developed countries. Each year, new evidence contributes to our knowledge on the role of breastfeeding on survival, growth and child development, as well as its benefits reaching adulthood. Our interest was to study if an appropriate breastfeeding protects against mammary carcinogenesis in adult life, for this we used Sprague Dawley female rats that at 24 hours of age were divided into two groups: one holding eight pups per mother and the other holding three, in order to generate greater exposure to breast milk. After weaning, half of the offspring from each group were fed with standard pet food (12.6% fat) and half with a high fat diet (40% fat). The latter was prepared by adding 12.1 g. of fat removed from the first bovine juice per 100g of food standard. At 55 days of age all rats were treated with an oral dose of DMBA (po, 15 mg / rat). The animals were weighed weekly to develop the respective growth curves and observed until the appearance of the first palpable tumor. Latency, incidence, growth rate and multiplicity were recorded. Furthermore intraabdominal fat content was analized and histological and immunohistochemical studies of the tumors were performed. Our results showed that tumoral incidence was lower (p