SPINK7 expression changes accompanied by HER2, P53 and RB1 can be relevant in predicting oral squamous cell carcinoma at a molecular level

PENNACCHIOTTI, G.; VALDES GARRIDO, F.; GONZÁLEZ ARRIAGA,W.; MONTES, H.; PARRA, J.; GUIDA, V.; GÓMEZ, S.E.; GUERRERO-GIMÉNEZ, M.E.; FERNANDEZ-MUÑOZ, J.M.; ZOPPINO, M.; CARÓN, R.W.; EZQUER, M.E.; FERNÁNDEZ RAMIRES, R.; BRUNA, F. A.

Scientific Reports

Nature Publishing Group

Año: 2021 vol. 11

The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 in precancerous lesions could promote oncogenesis. Our aim was to evaluate SPINK7 as a potential molecular biomarker which predicts OSCC stages, compared to: HER2, TP53, RB1, NFKB and CYP4B1. This study used oral biopsies from three patient groups: dysplasia (n = 33), less invasive (n = 28) and highly invasive OSCC (n = 18). The control group consisted of clinically suspicious cases later to be confirmed as normal mucosa (n = 20). Gene levels of SPINK7, P53, RB, NFKB and CYP4B1 were quantified by qPCR. SPINK7 levels were correlated with a cohort of 330 patients from the TCGA. Also, SPINK7, HER2, TP53, and RB1, were evaluated by immunohistofluorescence. One-way Kruskal?Wallis test and Dunn´s post-hoc with a p