INVESTIGADORES
BOLZAN Alejandro Daniel
artículos
Título:
Clastogenic effects of streptozotocin on human colon cancer cell lines with gene amplification
Autor/es:
ALEJANDRO DANIEL BOLZAN; MARTHA S. BIANCHI
Revista:
JOURNAL OF ENVIRONMENTAL PATHOLOGY, TOXICOLOGY AND ONCOLOGY : OFFICIAL ORGAN OF THE INTERNATIONAL SOCIETY FOR ENVIRONMENTAL TOXICOLOGY AND CANCER.
Editorial:
BEGELL HOUSE INC
Referencias:
Año: 2003 vol. 22 p. 281 - 286
ISSN:
0731-8898
Resumen:
We investigated the clastogenic effects of the methylating agent streptozotocin (STZ) on two
human colon cancer cell lines. COLO320DM and COL0O20HSR are cell lines derived from
a human neuroendocrine colon carcinoma. STZ produced a dose-dependent increase in the
frequency of chromosomal aberrations in both cell lines (p < 0.05) and induced fragmentation
and/or pulverization of COLO320DM and COLO320HSR chromosomes. This effect
was dose and time dependent. Severe chromosome damage was also found in cells that had
progressed beyond the first metaphase, and a higher percentage of metaphases showing pulverized
chromosomes were found in cells after the second than after the first division. This seems
to indicate that STZ has a persistent and delayed clastogenic effect on COLO320DM and
COLO320HSR cells. In addition, STZ produced a marked depression of the mitotic index in
both cell lines. These results demonstrate that human colon cancer cell lines COLO320DM
and COLO320HSR are highly sensitive to STZ, and suggest that this antibiotic has a good
potential as a chemotherapeutic agent for colon cancer.