CONICET | Buscador de Institutos y Recursos Humanos

Ethnopharmacological relevance:Geoffroeadecorticans (chañar) fruits and their derivate product(arrope)have been traditionally used as food and a folk medicine for the treatment of a wide variety of diseases including bronchopulmonary disorders and to relieve dolorous process. Aim ofthestudy: In order to evaluate the pharmacology action of this plant,studies were performed of antinociceptive and antioxidant activities. Materialsandmethods: The aqueous and ethanolic extracts and arrope of chañar were evaluated in various established pain models,including chemical nociceptioninducedbysubplantarformalinand intraperitonealaceticacidandthermalnociceptionmethod,suchastailimmersiontestinrats. To examinethepossibleconnectionoftheopioidreceptortotheantinociceptiveactivityofextractsand arrope itwasperformedacombinationtestwithnaloxone,anon-selectiveopioidreceptorantagonist. Results: The aqueousextractandarrope(1000mg/kg)causedaninhibitionofthepaininformalintest in thefirstphase,similartomorphineanddecreaseinthesecondphase.Inacombinationtestusing naloxone,diminishedanalgesicactivityofaqueousextractandarropewereobserved,indicatingthat antinociceptiveactivityisconnectedwiththeopioidreceptor.Theaqueousextractandarrope,caused an inhibitionofthewrithingresponseinducedbyaceticacid.Centralinvolvementinanalgesicprofile was confirmedbythetailimmersiontest,inwhichtheaqueousextractandarropeshowedasignificant analgesicactivitybyincreasinglatencytime.Theaqueousextractshowedhigherantioxidantactivity than thearrope,itmaybeduetothecookingprocess. Conclusions: This studyhasshownthattheaqueousextractandarropeof Geoffroeadecorticans (chan ar) fruits,doespossesssignificantantinociceptiveeffects.Itisfurtherconcludedthataqueousextractwith maximuminhibitionoffreeradicalisthemostpotentextractamounttestedextracts.Attheoraldoses tested theaqueousextractandarropewerenon-toxic.Thepresentresultsjustifiestheirpopularuse and constitutesthefirstvalidationstudyoftheantinociceptiveaction.