Metaprofiling of the bacterial community in sorghum silages inoculated with lactic acid bacteria

PUNTILLO, M.; PERALTA, G.; BURGI, M; HUBER, P.; GAGGIOTTI, MÓNICA; BINETTI, A. G.; VINDEROLA, C. G.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2022

1364-5072

Aims: to characterize the fermentation process and bacterial diversity ofsorghum silage inoculated with Lactiplantibacillus plantarum LpAv, Pediococcuspentosaceus PpM and Lacticaseibacillus paracasei LcAv. Methods and Results: chopped sorghum was ensiled using theselected strains. Physicochemical parameters (Ammonia Nitrogen/Total Nitrogen,Dry Matter, Crude Protein, Acid Detergent Fiber, Neutral Detergent Fiber, AcidDetergent Lignin, Ether Extract and Ashes), bacterial counts, cell cytometryand 16sRNA sequencing were performed to characterize the ensiling process andan animal trial (BALB/c mice) was conducted in order to preliminary explore thepotential of sorghum silage to promote animal gut health. After 30 days ofensiling, the genus Lactobacillus comprised 68.4 ± 2.3 % and 73.5 ± 1.8% of relative abundance, in control and inoculated silages respectively.Richness (Chao1 index) in inoculated samples, but not in control silages,diminished along ensiling, suggesting the domination of fermentation by theinoculated LAB. A trend in conferring enhanced protection against Salmonellainfection was observed in the mouse model used to explore the potential topromote gut health of sorghum silge. Conclusions: the LAB strains used in this study were able to dominate sorghumfermentation. Significance and Impact of the Study: this is the first reportusing metaprofiling of 16sRNA to characterize sorghum silage, showing amicrobiological insight where resident and inoculated LAB strains overwhelmedthe epiphytic microbiota, inhibiting potential pathogens of the genus Klebsiella.Keywords: sorghum, silage, NGS, profiling, inoculants, L.plantarum, L. paracasei, P. pentosaceus. @font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-520082689 -1073697537 9 0 511 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-fareast-language:EN-US;}div.WordSection1{page:WordSection1;}