Endothelin 1 impairs norepinephrine neutornal transporter in PC12 cells. Role of the endoplasmic reticulum stress.the

FABIÁN INNAMORATO COSTAS; NICOLÁS CIANGRECO; GUADALUPE ALVAREZ; ANA PAULA COURREGES; CLARISA MAROTTE; LILIANA G. BIANCIOTTI; MARCELO S. VATTA

Mar del Plata

Congreso; Reunion Anual de Sociedades Biomédicas; 2022

Sociedad Argentina de Investigación Clínica

Neuronal norepinephrine transporter (NET) removes norepinephrinefrom the synaptic cleft. Several studies show that NET impairmentis associated with cardiovascular diseases like hypertension(HT) whereas recent works link endoplasmic reticulum stress (ERE),triggered by misfolded proteins accumulation in the reticulum, withthe development of HT. We recently reported that endothelin receptors(ETA/ETB) blockade in rats with salt-dependent HT decreasesblood pressure and markers of ERE and ERE-dependent apoptosisas well as misfolded NET accumulation in the adrenal medulla. Inthe present study we aimed to unveil the mechanisms underlyingthese findings. PC12 cells were exposed to 10mM endothelin 1 (ET-1), 10nM thapsigargin (TG) or 0.1ug/ml tunicamycin (TN) (TG andTN were used as ERE positive controls) and NET and ERE markersexpression were assessed by Western blot. Results were expressedas the mean ±SD, and p