On How Conformation Of Biliverdin Influences Their Reduction To Bilirubin: A Biological and Molecular Modeling Study.

MORA, MARÍA EUGENIA; BARI, SARA E.; AWRUCH, JOSEFINA; DELFINO, JOSÉ MARÍA

Bioorganic & Medicinal Chemistry

Elsevier

Lugar: Amsterdam; Año: 2003 vol. 11 p. 4661 - 4672

0968-0896

The cyclic 2,18-bridged biliverdin (2) is excreted in rat bile without reduction to the corresponding bilirubin. Conformational analysis, employing an optimized Monte Carlo method and a mixed Monte Carlo/stochastic dynamics, reveals that biliverdin IXα (1) and the cyclic analogue 2 adopt ‘lock washer’ conformations, stabilized by the presence of intramolecular hydrogen bonds between N23…H22N and, to a lesser extent, between N23…H24N. Although 2 is very similar in overall shape to 1, the former adopts a ‘locked lock washer’ conformation unable to undergo fluctuations, thus possibly hampering a proper recognition by biliverdin reductase.