Bordetella Pertussis Pertactin Deficient Clinical Isoltes Were Not Detected In Whole Cell Vaccinated Population From Argentina.

CARRIQUIRIBORDE F; BARTEL E; BOTTERO D; BRAVO S; HOZBOR D

Simposio; 11 th Internation Bordetella Symposium; 2016

BORDETELLA PERTUSSIS PERTACTIN DEFICIENT CLINICAL ISOLTES WERE NOT DETECTEDIN WHOLE CELL VACCINATED POPULATION FROM ARGENTINACARRIQUIRIBORDE F; BARTEL E; BOTTERO D; BRAVO S; HOZBOR DLaboratorio VacSal. Instituto de Biotecnología y Biología Molecular. FCE-UNLP-CONICET. La Plata ArgentinaA recent increase in Bordetella pertussis without the pertactin protein, an acellular vaccine immunogen, has beenreported in several countries that only used acellular vaccine in the calendar. Determining whether pertactin-deficient(PRN(-)) B. pertussis is also present in whole cell vaccinated population is needed.ObjectiveMolecular characterization of Bordetella pertussis clinical isolates obtained during 1969-2014 period in Argentina,a country where whole cell vaccines are used for primary doses.Materials and MethodsThree hundred and twenty two isolates of B. pertussis were mainly obtained at our laboratory within the surveillanceof pertussis in Argentina between 2000 and 2014. Nine isolates obtained during 1969 and 1998 (oldisolates) were also included. To detect the appearance of B. pertussis pertactin deficient isolates, molecular PCRbasedmethodology and immunoblotting with specific pertactin sera were used.ResultsIsolates were screened for the prevalent mutations (IS481 insertion or deletion in prn gene) that have been documentedto cause pertactin deficiency by previously reported polymerase chain reaction amplification methods.42 | 11th International Bordetella SymposiumNeither IS481 insertion nor prn gene deletion were detected in all isolates analyzed. To perform the immunoblotassays a subset of 20 isolates harboring prn1 or prn2 allele were selected. From this subset, only 1 prn1 isolatefrom 2006 expressed PRN in a very low level.ConclusionsThe results presented here are in agreement with the hypothesis that pertactin-deficient B. pertussis isolatescirculate only in acellular vaccinated population