Poly(allylamine)-tripolyphosphate self-assemblies: Towards robust and biocompatible drug nanocarriers

PICCO, A.S.; PADULA, G.; CABRERIZO, F.; HERRERA, S.; SEOANE, A.; CORTEZ, L.; AGAZZI, M.; ALOMAR, L.; AZZARONI, O.

Virtual

Conferencia; Virtual Vancouver Nanomedicine Day 2020; 2020

NMIN

Nano-sized self-assemblies produced through ionic crosslinking of polyelectrolytes withmultivalent ions are attractive platforms for delivery of drugs, genes, and imaging agents. Theyare formed under mild conditions and can be easily loaded with cargo molecules. Moreover,owing to the wide spectrum of available building blocks (polyelectrolytes and crosslinkers), theycan be designed for responding to different external stimuli such as pH, temperature, light, redox,among others.1,2,3 However, lack of stability during storage or when dispersed in relevant biologicalfluids combined with insufficient toxicological characterization, limits their ultimate application.4Here we present the advantages of poly(allylamine) (PAH) ? tripolyphosphate (TPP) selfassemblies. Monodisperse PAH-TPP colloidal complexes of ca. 200 nm (as seen by TEM and DLS)were prepared by a simple one-pot procedure, showing long-time stability (> 6 months) andcapacity to be dispersed in complex media (DMEM + FBS) without aggregation. Moreover, theydid not exhibit cytotoxicity in A549 cells, neither genotoxicity (as confirmed by comet andmicronucleus assays), nor hemolytic activity. Owing to these remarkable features, PAH-TPP selfassemblies are promising candidates for using as stable and safe drug nanocarriers.