Rsb and FST analysis revealed selection swifts in hypoxia-inducible factor genomic region on Bolivian highland Creole cattle

PEREIRA RICO J.A.C.; FERNANDEZ M.E.; ALVAREZ CECCO P.; LOZA VEGA A.; ROGBERG MUÑOZ A.; FALOMIR LOCKHART A.H.; ARCE CABRERA O.N.; GIOVAMBATTISTA G.

Mendoza

Congreso; XVII CONGRESO LATINOAMERICANO DE GENÉTICA; 2019

Asociación Latinoamericana de Genética

Latin-American Creole cattle are spread all over American countries and are descendants of the bovines introduced into America by the Spanish and Portuguese conquerors in the 16th century. Most of populations evolved under low levels of breeding management and became adapted to different environments, such as tropical rainforest, subtropical dry forest, highland steeps. In Bolivia, some populations have adapted to the Andean highland plains at 4000 m.a.s.l., which probably created signatures of selection within the actual genomes. The aim of this study was to study the footprints of adaptation to altitude in a population of Bolivian Creole cattle. 132 animals (67 highland and 65 lowland) were genotyped using ArBos1 50K microarray. Quality control was performed considering 97% as sample and SNP call rates and MAF values. Shape-IT software was used to determine the individual haplotypes and the rehh package from R was applied to compute Rsb. Plink v1.9 was used to estimate pairwise FST between populations. The top 1% values from both indexes were taken as threshold to consider significant results and BovineMine database was used to retrieve the genes. The most significant results were found on BTA10 and 20, which showed significant peaks through both methods. Within BTA10 peak, we detected a gene, HIF1 (hypoxia inducible factor 1), that functions as transcriptional regulator of the adaptive response to hypoxia, and was previously associated to altitude adaptation.