Effect of Different Fatty Acid Profiles in the Maternal and Finishing Diet on Liver Fatty Acid Profile and Gene Expression

A E RELLING; D N COLEMAN; A C CARRANZA MARTIN

Congreso; 2018 ASAS ADSA Midwest Meeting; 2018

line"><The objective of this study was to evaluate the effectsof eicosapentaenoic acid (EPA) and docosahexaenoicacid (DHA) supplementation to ewes during late gestation on fiishing lamb liver tissue (LT) fatty acid (FA)profie and gene expression. Lambs born from ewessupplemented with Ca salts of the PUFA EPA andDHA, or palm FA distillate (PFAD) high in palmiticand oleic acid at 0.39% DM during the last 50 d ofgestation were used. Lambs were weaned at 60 d ofage and adapted to a high concentrate diet for 1.5 mo.After adaptation, 74 Hampshire x Dorset cross lambs(28 pens) were blocked by initial BW when the fiishingperiod started and used in a 2x2 factorial arrangementof treatments using the factors of dam diet and lambdiets containing PUFA or PFAD at 1.5% DM. Lambswere slaughtered after 42 d of supplementation andLT samples were obtained for FA analysis and geneexpression analysis of 27 genes. A lamb x dam treatment interaction (P=0.01) was observed for the omega-6:omega-3 FA ratio; lambs that were supplementedwith PUFA and born from PFAD ewes had the lowestratio, and lambs born from PFAD ewes and supplemented with PFAD during the fiishing period had thehighest ratio. Concentrations of EPA and DHA in lambliver were signifiantly greater (P<0.001) with PUFAsupplementation vs. PFAD supplementation duringthe fiishing period, but there was no effect of dam diet(P>0.10). Only one signifiant dam effect was observedfor cis-9 C18:1 (P=0.05), where concentrations weregreater in lambs from PFAD ewes. There were nolamb x dam treatment interactions observed for the 27genes measured (P>0.10). A signifiant dam effect wasobserved for hormone sensitive lipase (P=0.02), wheremRNA expression was greater in lambs from PUFAsupplemented ewes. Signifiant lamb treatment effectswere observed for stearoyl-CoA desaturase (P<0.001),fatty acid synthase (P<0.001), Δ 5-desaturase (P<0.001)and Δ 6-desaturase (P<0.001), where mRNA expressionwas decreased with PUFA compared to PFAD supplementation during the fiishing period. Expression oflipoprotein lipase (P  =  0.01) was decreased in PUFAvs. PFAD lambs. These changes in mRNA expressionsuggest that lipogenesis may be decreased and lipolysis increased in lamb LT with PUFA vs. PFAD supplementation during the fiishing period. These resultssuggest that little interaction exists between FA supplementation of dams during late gestation and supplementation of FA to lambs during the fiishing periodon liver FA and gene expression.