Morphological techniques used in ichthyopathological diagnosis

ANDRÉS LAUBE, P.F.; MOYANO, D.A.; PLAUL, S.E.; SANTAMARÍA MARTÍN, C.J.; PACHECO MARINO, S. G.; BARBEITO, C.

Microscopy and imaging science: practical approaches to applied research and education

FORMATEX Microscopy Book Series Nº 7

Lugar: Badajoz; Año: 2017; p. 269 - 280

In this review, different diagnostic techniques for identifying etiology of fish disease are described. Among microscopictechniques, we find rapid diagnostic tests to observe parasites, gill diseases and bacteria; routine histopathologicaltechniques are also used. In samples having bone tissue, decalcification methods are employed. In recent years, apart fromusing immunohistochemistry and electron microscopy, morphological techniques allow a deep study of diseasepathogenesis. At present, molecular techniques for pathogen identification and atomic-absorption spectrophotometry tomeasure the concentration and distribution of toxic metals in tissues they also use. In conclusion, different techniques cancontribute to the evaluation of disorders and provide tools for a better understanding in fish medicine.