Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta.

MARIANA RECAVARREN; SILVIINA QUINTANA; RAMIRO DESANTADINA; ALEJANDRO ENRIQUE RELLING

Biosci. J., Uberlândia

Oswaldo Marcal Junior, UFU

Lugar: Umuarama; Año: 2018 vol. 34 p. 180 - 185

1981-3163

The objective of the study was to evaluate the effect of time of gestation on fatty acid transportermRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles)and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles andcotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data wereanalyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) withrepeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereasanimal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to agreater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. Onthe second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similarconcentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were notdifferent (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetaldevelopment.