IGEVET 21075
INSTITUTO DE GENETICA VETERINARIA "ING. FERNANDO NOEL DULOUT"
Unidad Ejecutora - UE
artículos
Título:
Development of an ELA-DRA gene typing method based on pyrosequencing technology
Autor/es:
DIAZ S; ECHEVERRIA MG; IT V; POSIK DIEGO; ROGBERG MUÑOZ ANDRÉS; PENA N; PERAL GARCÍA PILAR; GIOVAMBATTISTA GUILLERMO
Revista:
TISSUE ANTIGENS
Editorial:
Blackwell Publishing
Referencias:
Año: 2008 vol. 72 p. 464 - 468
ISSN:
0001-2815
Resumen:
Molecular typing of the Equine Lymphocyte Antigen (ELA) class II DRA gene by Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) and Reference Strand-Mediated Conformation Analysis (RSCA), allow identifyingeleven ELA-DRA exon 2 sequences, three of which are widely distributed among domestic horse breeds. Herein, we describe the development of a pyrosequencing-based method applicable to ELA-DRA typing, by screening samples from eight different horse breeds previously typed by PCR-SSCP. This sequencebased method would be useful in high throughput genotyping of MHC genes in horses and other animal species, making this system interesting as a rapid screening in animal genotyping of immune related genes.
