Adenosine regulates cytotoxic CD4 T lymphocytes in Trypanosoma cruzi infection

BERGERO, G; MAZZOCCO, YL; EBERHARDT, N; AOKI, MP

Congreso; Reunión Conjunta SAIC SAI AAFE NANOMED. Noviembre 2021.; 2021

ADENOSINE REGULATES CYTOTOXIC CD4 T LYMPHOCYTES IN TRYPANOSOMA CRUZI INFECTIONAuthors:Bergero Gastón1; 2, Mazzocco Yanina1; 2, Eberhardt Natalia3, Aoki Maria Pilar1; 2.1Departamento de Bioquímica Clínica e Inmunología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI)-CONICET, Córdoba, Argentina. 3Department of Medicine, Leon H. Charney Division of Cardiology, New York University School of Medicine, New York.AbstractGrowing evidence demonstrates a critical role of purinergic system in the modulation of the immune response and to determine the outcome of infections. Damaged cells release the pro-inflammatory molecule ATP, which is metabolized by the ectonucleotidases CD39 and CD73 to the anti-inflammatory mediator adenosine (ADO).The mechanisms involved in the induction of CD4 T cell population with cytotoxic effector functions (CD4 CTL) have not been evaluated in the setting of experimental T. cruzi infection. Here, we explored the effect of CD73 activity abrogation (CD73KO) on the differentiation and functional capacity of the CD4 CTL population in T. cruzi infected mice.Through flow cytometry, we found that expression of multiple effector molecules (granzyme B, perforin, IFN- and TNFα) and the degranulation marker, CD107a, were increased in CD4 T spleen cells of CD73KO mice compared to WT mice at 14 days post-infection (dpi) (p