INVESTIGADORES
ALVAREZ Hector Manuel
artículos
Título:
Glycogen formation by Rhodococcus species and effect of inhibition of lipid biosynthesis on glycogen accumulation in R. opacus PD630
Autor/es:
HERNANDEZ MA; ALVAREZ, HÉCTOR M
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. - p. 93 - 99
ISSN:
0378-1097
Resumen:
Members of the genus Rhodococcus were investigated for their ability to produce
glycogen during cultivation on gluconate or glucose. Strains belonging
to Rhodococcus ruber, Rhodococcus opacus, Rhodococcus fascians,
Rhodococcus erythropolis and Rhodococcus equi were able to produce
glycogen up to 0.25.6% of cellular dry weight (CDW). The glycogen
content varied from 0.8% to 3.2% of CDW in cells of R. opacus PD630,
which is a well-known oleaginous bacterium, during the exponential
growth phase, when cultivated on diverse carbon sources. Maltose and
pyruvate promoted glycogen accumulation by cells of strain PD630 to a
greater extent than glucose, gluconate, lactose, sucrose or acetate.
This strain was able to produce triacylglycerols, polyhydroxyalkanoates
and glycogen as storage compounds during growth on gluconate, although
triacylglycerols were always the main product under the conditions of
this study. Cerulenin, an inhibitor of de novo fatty acid synthesis,
inhibited the accumulation of triacylglycerols from gluconate and
increased the content of polyhydroxyalkanoates (from 2.0% to 4.2%, CDW)
and glycogen (from 0.1% to 3.0%, CDW). An increase of the
polyhydroxyalkanoates and glycogen content was also observed in two
mutants of R. opacus PD630, which produced reduced amounts of
triacylglycerols during cultivation of cells on gluconate.