INVESTIGADORES
ALVAREZ Hector Manuel
artículos
Título:
Glucosamine-P and rhodococcal ADP-glucose pyrophosphorylases: A hint to (re)discover (actino)bacterial amino sugar metabolism
Autor/es:
CEREIJO, ANTONELA E.; ALVAREZ, HECTOR M.; IGLESIAS, ALBERTO A.; ASENCION DIEZ, MATIAS D.
Revista:
BIOCHIMIE
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Lugar: Paris; Año: 2020 vol. 176 p. 158 - 161
ISSN:
0300-9084
Resumen:
Glycogen was described as a temporal storage molecule in rhodococci, interconnecting lipids and carbonavailability. The Rhodococcus jostii ADP-glucose pyrophosphorylase (ADP-GlcPPase) kinetic and regulatoryproperties support this role. Curiously, the enzyme uses glucosamine-1P as alternative substrate.Herein, we report the in-depth study of glucosamine-1P activity and its regulation in two rhodocoocalADP-GlcPPases, finding that glucosamine-6P (representing a metabolic carbon/nitrogen node) is a criticalactivator, then reinforcing the role of glycogen as an ?intermediary metabolite? in rhodococci.Glucosamine-1P activity in rhodococcal ADP-GlcPPases responds to activation by metabolites improvingtheir catalytic performance, strongly suggesting its metabolic feasibility. This work supports a scenariofor new molecules/metabolites discovery and hypothesizes on evolutionary mechanisms underlyingenzyme promiscuity opening novel metabolic features in (actino)bacteria.
