Hygromycin resistance as an efficient selectable marker

ORTIZ, J; REGIARDO, M; RAVIZZINNI, R; ALTABE, SILVIA GRACIELA; CERVIGNI, G; SIPTELLER, M; VALLEJOS, R

PLANT CELL REPORTS

SPRINGER

Lugar: Berlin; Año: 1996 vol. 15 p. 877 - 881

0721-7714

A highly efficient method for stable wheat transformation using hygromycin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder-driven microparticle accelerator. Transgenic wheat plants were determined by PCR amplification of transgene fragments and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relative to the number of bombarded caUi) of 5.5% compared with 2.6% for the bar gene.