Expression of a chromosomal virulence region of Agrobacterium tumefaciens in Azospirillum spp

ALTABE, SILVIA GRACIELA; IÑON DE IANNINO, N; DE MENDOZA, D; UGALDE, R

JOURNAL OF BACTERIOLOGY

AMER SOC MICROBIOLOGY

Lugar: Baltimore, Whashington; Año: 1990 vol. 172 p. 2563 - 2567

0021-9193

nner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize beta-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of beta-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasilense strains carrying a cosmid containing the chromosomal virulence genes chvA and chvB of Agrobacterium tumefaciens formed beta-(1-2) glucan in vitro and synthesized the 235-kilodalton intermediate protein. No DNA homology to the chvB region was found in different wild-type strains of A. brasilense, but the introduction of a cosmid containing the Agrobacterium tumefaciens chvA and chvB regions yielded strains in which DNA hybridization with the chvB region was detected, provided that the strains were grown under an antibiotic selective pressure.