Morphological comparison of in vitro cultivated and wild Hedeoma multiflora Benth plants

LEPORATI JORGE; MAGALLANES NOGUERA CYNTHIA; CECATI FRANCISCO MIGUEL; KURINA SANZ MARCELA; DIAZ GABUTTI MARIA SOLEDAD; VERDES PATRICIA

Simposio; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2021

Hedeoma multiflora Benth. (Lamiaceae) known as "peperina de las lomas" is a perennial herb showing flavoring, digestive and antirheumatic properties. Native to temperate America, it is found in the central mountains of Argentina, mainly in San Luis and Córdoba. Due to its medicinal uses, along with the expansion of agricultural and urban border, it is very depressed and the survival of the natural populations is in danger. We consider in vitro culture as an alternative to ensure H. multiflora germplasm preservation and its sustainable use. The aim of this work is to compare the exomorphology of wild specimens collected in three different sampling sites (S1: between El Morro and Villa Mercedes 33° 17 25?S 65°28 07? W; S2: La Esquina, El Morro 33°11 03? S 65° 22 08? W and S3: Dique Boca del Río, Merlo 32° 58 37.8? S 65°02 50.4? W) toward two populations of micropropagated plants in vitro, to evaluate the effect in vitro organogenesis. The number and weight of the stems and the main roots were measured. Statistical analyzes were carried out with the R Studio program. The repetitions were ten for each site and population tested. The analysis was performed with the Kruskal Wallis statistic and the differences between the average measurements were considered significant at a value of p