INTEQUI   20941
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Biotransformation of dihydrocoumarin by Aspergillus niger ATCC 11394
Autor/es:
AGUIRRE PRANZONI, C.; FURQUE, G.I.; ARDANÁZ, C.E.; PACCIARONI, A.; SOSA, V.; TONN, C.E.; KURINA SANZ, M.B.
Revista:
ARKIVOC - ONLINE JOURNAL OF ORGANIC CHEMISTRY
Editorial:
ARKAT USA INC
Referencias:
Año: 2011 vol. 2011 p. 170 - 181
ISSN:
1551-7004
Resumen:
Abstract
Aspergillus niger ATCC 11394 was used to catalyze the
biotransformation of dihydrocoumarin using two experimental conditions: growing
and resting cells. Six biotransformation products (2-7) were purified from the
bio-reaction media and their structures elucidated by spectroscopic methods and
mass spectrometry. The latter technique coupled to gas chromatography was used to
analyze the time course of the biotransformations; from which results a
metabolic pathway was proposed. The use of cytochrome P450 enzyme inhibitors,
as well as working under poor oxygen conditions allowed us both to inquire
about the type of enzymes involved in the process, and to design methods to
prepare only metabolites that do not come from biological oxidations.