Alternative technique for dosing of digoxin in dissolution samples

DI CHIACCHIO GABRIELA; GOMEZ ROXANA; VICARIO, ANA; SOLARI VICTOR MANUEL; ARAGÓN, LESLIE M; CHIEN C WANG

CORDOBA

Congreso; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); 2021

Digoxin is a type of medicine called a cardiac glycoside that is used to control some heartproblems, such as irregular heartbeats (arrhythmias) including atrial fibrillation.This drug ischaracterized by a narrow therapeutic range (0.5?2.0 ng mL ?1 in serum). Considering this levelconcentration, in order to perform pharmacokinetics, bioequivalence or therapeutic drug monitoringstudies, it is necessary to develop sensitive and selective methods.Dissolution testing is a routine quality control, that also provides substantial datafor the estimation ofthe bioavailability of solid oral dosage forms. Quantification of digoxin in the dissolution samples isbased on measurement of fluorescence induced by exposing the drug to drastic conditions.The objective of this work is to develop a simple HPLC-UV method for the determination of digoxinafter dissolution without derivatization, by using molecularly printed polymers (MIP) for improvingthe selectivity and sensitivity. Satisfactory results were obtained retaining specifically digoxin(92.3%).This methodology allows the pre concentration of digoxin 10 times after dissolution testing, withoutfluorescence detection in a fast way with low solvent consumption, reuse of the polymers andminimum cost and difficulty of work. They were achieved limits of detection in order to 0.15µg/ml.