IMBALANCE IN HOMEOSTASIS RAT PLACENTA EXPOSED TO CADMIUM (G20). EFFECTS OF SOY PROTEIN AS A PROTEIN SOURCE

PEREZ CHACA MV; CIMINARI E; ALVAREZ SM; PIGUILLEM SN; BOLDRINI G; BIAGGIO VS; FILIPPA V; GOMEZ NN

Mar del Plata

Congreso; Sociedad Argentina de Investigacion Clinica; 2019

Sociedad Argentina de Investigacion Clinica

Introduction: Autophagy and apoptosis are two crucial and interconnected processesin the placenta that are often influenced by oxidative stress. Alteration between the protective anddestructive mechanisms of autophagy and apoptosis seems to be associated topregnancy-related disorders. In addition, Cd canpass through the placenta and accumulates in fetal tissues, so that rat fetaltoxicity is caused by placental or maternal Cd-induced dysfunction, not by a Cddirect effect on fetuses. On the other way, soy protein is becoming increasinglyimportant in the human diet. Isoflavones(genistein) could cause hypertrophy in endometrium and alter reproductivefunction in different species. Objective: to evaluate the possibleprotective role of soy protein consumption compared to the mechanisms bywhich Cd exerts its toxicity. Materials and Methods: 4 lots of female Wistar rats were used: 2 lotsreceived casein (Cas) and 2 lots soybean (Soy) as protein source. Within eachgroup, 1 lot received regular water (Co) and the other, 15 ppm of Cd in thedrinking water. We determined TBARS, CAT and GPx activity, andnitrite concentration. RT-PCR was performed using the following primers: MT I; MT II; Nrf-2; NOX-2, SOD-2 and CAT. Results:In Soy-Cd group Nrf-2,SOD and MT I expression increased (p