Morphological and lipid metabolism alterations in mammary gland of vitamin A deficient rats

VASQUEZ ME, CARMONA Y, JAHN G, GIMENEZ MS

San Luis

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2009

Sociedad de Biología de Cuyo

Morphological and lipid metabolism alterations in mammary gland of vitamin A deficient rats Vasquez ME, Carmona Y, Jahn G, Gimenez MS Dept Biochemistry and Bbiological Cs. UNSL. IMIBIO-SL San Luis CONICET 5700 Email: eridnere@hotmail.com We have observed that vitamin A alters lipid metabolism and morphology of liver, heart and aorta. Our objective was determined if the vitamin A deficiency, alter the mammary gland metabolism, through different parameters. Wistar female rats were separated at weaning into 3 groups. One was fed with diet sufficient in vitamin A (A +,) another with a diet deficient in vitamin A (A.) and the third group  receive Vitamin A deficient diet for 75 days after of which they received vitamin A sufficient diet for 15 days (Ar). The morphology was performed by optical microscopy and lipids were extracted by method of Folch-and post-TLC separation of the different fractions. (A+) have developing virgin rats. (A-) has ductal development more than (A+) and appears to have an incipient lobe alveolar up growth. In (Ar) the lobe alveolar development had modest but much more than the (A-).We observed that decreased of total phospholipids in (A-) p<0.001 in relation to (A+) and (Ar). There were not differences in the amount of cholesterol and triglycerides between the three groups. The deficiency of vit A modified the morphology and phospholipids content in mammary gland.