Adrenergic agents modify ovarian p450 aromatase expression in the rat first oestral cycle.

GORDILLO M; DELGADO SM; CARRIZO D; ANZULOVICH AC; RASTRILLA AM

Huerta Grande, Cordoba

Congreso; I IRCN de la Sociedad Argentina de Investigación en Neurociencias y de la TAN; 2009

We have showed that peripheral innervation participate in ovarian oestradiol release. The purpose of this work was to analyze the effects of Norepinephrine (NE), ¥á and ¥â adrenergic antagonists, Phentolamine (PHE) and Propranolol (PROP) respectively in coeliac ganglion (GC) on the ovarian release of androstenedione (A2) and P450arom expression during the first proestrous (PE), estrous (E) and diestrous (D) in rat. We used the ex vivo GC-superior ovarian nerve-ovary system. Witch was incubated in Krebs Ringer at 37¨¬C in atmosphere of 95O2-5CO2. The groups were: a- Control, b, c and d, NE, PHE and PROP at the same concentration 10-6 M. Aliquots from the ovarian compartment were taken at 15, 30, 60 and 120 min. A2 was measured by RIA. After 120 min, ovaries were separated and the total RNA was extracted by the method of TRIzol and levels of P450arom mRNA were semiquantified using RT-PCR. Was utilized ANOVA 1 followed by Tuckey test with a significance of p0.05. The results shown that in PE, PROP decreased ovarian A2 release at 30 min. In E, NE increased A2 release at 60 min and decreased its levels at 30 min, while PHE increased A2 at 30 and 60 min and PROP at 60 and 120 min. In D, NE and PHE increased ovarian A2 levels at all studied times. Ganglionic NE addition would have a differential effect on the P450arom mRNA enzyme expression depending on the oestral cycle stage. FEN stimulation antagonized adrenergic effects only in PE, where P450arom mRNA control levels are higher in comparison to E and D. The results suggest that the mechanism of adrenergic ganglion stimulation underlying on ovarian steroidogenesis would be, at least in part, mediated by ¥á and/or ¥â adrenergic receptors.