DIETARY SOY-BASED PROTEIN MODULATES THE OXIDATIVE AND INFLAMMATORY EFFECTS OF CADMIUM IN HIPPOCAMPUS

PLATEO PIGNATARI MG; MARTIN MOLINERO GD; BOLDRINI GG; GIMENEZ MS; DELLA VEDOVA MC; RAMIREZ DC

Cordoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Oxidative stress and inflammation can change hippocampus (Hp) biochemistry and physiology. Cadmium (Cd) is a neurotoxicant, but its effects on Hp are rarely informed and whether these effects can be modulated by the diet is unknown. Herein we hypothesize that low doses of Cd in the drinking water causes oxidative/inflammatory changes in the Hp and that soybean-protein-based diet may modulate these effects. To accomplish this goal, we fed 4 groups of female Wistar-rats for 60 days as follows: 1:casein-based diet (CBD) + tap water; 2: CBD + tap water with 15 ppm Cd (as Cl2Cd); 3: soybean-based diet (SBD) + tap water; and 4: SBD + tap water with 15 ppm Cd. In the Hp of CBD group, Cd exposure did not change superoxide dismutase (SOD) activity, TNF-α concentration and myeloperoxidase (MPO) activity. In the serum of rats fed a SBD, Cd increased SOD activity, whereas reduced the TNF-α concentration. Compared to CBD, the Hp of SBD showed enhanced MPO activity?a marker of leukocyte inflammation. On the other hand, in the Hp of CBD rats Cd induced endothelial nitric oxide synthase (NOS) and inducible NOS expression?markers of inflammation. This effect was not observed in SBD rats. Together our data suggest that SBD may trigger a compensatory antioxidant and inflammatory mechanism in the Hp of rats chronically exposed to low concentration of Cd.