Vitamin A deficiency: alters oxidative stress and inflammation gene expression in mammary gland.

FERRARI VIVAS C. ; OROZCO AGUSTINA; VASQUEZ GOMEZ ME; GIMENEZ MS

Cordoba

Congreso; Reunion Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigacion Bioquímica y Biología Molecular

The objective of this study is to determine the effect of vitamin A deficiency (VAD) on oxidative stress and inflammation. Wistar female virgin rats were separated at weaning into 6 groups: fed with vitamin A sufficient diet for 3 months (c3m), vitamin A deficient diet for 3 months(d3m), vitamin A deficient diet for 75 days followed by refeeding with vitamin A sufficient for 15 days (r3m), vitamin A sufficient diet for 6 months (c6m), vitamin A deficient diet for 6 months (d6m) and vitamin A deficient diet for 150 days followed by refeeding with vitamin A sufficient for 30 days (r6m). The expressions of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), Nrf-2, iNOS, eNOS, NFκb, TNFα,Cox2 were measured by RT-PCR; and also TBARs and Carbonil levels.TBARS and Carbonil levels and CAT, SOD, GPx, Nrf2, iNOS and eNOS expression did not show differences between groups. The expression of NFκb, TNFα, Cox2 increased in d3m with respect to c3m and r3m (p