CADMIUM INDUCES HYPERTENSION, ALTERATIONS IN OXIDATIVE STRESS AND APOPTOSIS MARKERS TOGETHER WITH MORPHOLOGYCAL CHANGES IN AORTA

ALVAREZ SILVINA M; BOLDRINI GABRIEL GIEZI; MARTIN MOLINERO GLENDA D; BIAGGIO VERONICA S; PIGUILLEM SN; GOMEZ NN; GIMENEZ MS

Congreso; Reunión Conjunta de Sociedades de Biología de Argentina; 2015

Cadmium (Cd) is a toxic metal and an important environmental contaminant. We studied its effects on blood pressure, histoarchitecture, oxidative stress and apoptosis markers of rat aorta. Male Wistar rats were used: 1 group received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days (Cd). During the treatment, blood pressure was measured with a CODA system. Total RNA was isolated with Trizol and cDNA was obtained. Nrf2 factor, NOX2, GPx, SOD, p53, Transforming growth factor beta (TGF-β), BAX and Bcl -2 were determined by PCR. BAX/Bcl-2 ratio was calculated. S28 was used as control. Aortas were fixed, sectioned, stained, and examined for evidence of injury. Cd induced significant increase in systolic and diastolic blood pressure (p