CADMIUN ALTERS THE LIPID METABOLISM IN RAT MAMMARY GLAND. EFFECT OF SOY PROTEIN

SANCHEZ ES; VASQUEZ E; PLATEO G; ARNAL N; BOLDRINI GG; ÁLVAREZ SM; GIMÉNEZ MS

Mar del Plata

Congreso; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2015

We studied the effect of different diets on the lipid profile of rat mammary gland (MG). We also looked at thepotential protective outcome of those diets under exposure to Cadmiun (Cd), which is a toxic element and animportant environmental contaminant.4 female lots Wistar rats were used: 2 lots received casein (Cas) and 2 lotssoybean (Soy) as protein source. Within each group, 1 lot received regular water (control) and the other, 15 ppm ofCd in the drinking water for 60 days. Lipids were extracted by Hexano:Isopropanol:BHT( 3v:2v:1%.) solvent. Totalcholesterol (TC) and phospholipids (PL) were determined by colorimetric assay. Fatty acids (FA) were subjected togas chromatography-mass spectrometry. HMG CoA Reductase (HMGCoAR) protein expression was determined byWestern blot. Interestingly, our results show that Soy diet decreases TC and tend to increase PL in relation to Cas. Cddoes not influence the level of TC and PL in either group. HMGCoAR expression is decreased by Cd in CAS fed ratsas well as on both group of Soy diet. Regarding to the FA composition we observed that Cd decreases the amount of18:2 y 18:3n3 in CAS group, which may suggest that Soy prevents the effect of Cd. Simultaneously, Soy decreases16:0 compared to Cas. These findings indicate that Cd exposure alters the composition of lipids in MG and Soyprotein diet may regulate these effects.