WESTERN DIET-INDUCED OBESITY AND NEUTROPHIL-DRIVEN LUNG INLFAMMATION

DELLA VEDOVA,MC ; MUÑOZ, MD ; MORENO-SOSA, T; RINALDI TOSI , M; GARCIA, S; GOMEZ MEJIBA, SE; RAMIREZ, DC

San Luis

Congreso; XXXII; 2014

Sociedad Biologia de Cuyo

WESTERN DIET-INDUCED OBESITY AND NEUTROPHIL-DRIVEN LUNG INLFAMMATION Della-Vedova,MC 1,2 ; Muñoz, MD1,2 ; Moreno-Sosa, T1; Rinaldi Tosi , M1; Garcia, S3; Gomez Mejiba, SE1,2; Ramirez, DC.1,2 1Laboratorio de Medicina Experimental y Terapéuticas, IMIBIO-SL-CONICET-UNSL; 2Universidad Nacional de San Luis; 3IMIBO-SL, CONICET. E-mail: ramirezlabimibiosl@ymail.com Adipose tissue inflammation is the source of a number of proinflammatory mediators that affect insulin sensitivity and may also affect neutrophil traffic in the lung microvasculature. Myeloperoxidase (MPO) is the main protein of neutrophils and the only one that uses H2O2 to oxidize Cl- to HOCl?a powerful oxidant that causes protein chlorotyrosine (Cl-Tyr). Herein we aimed at determining whether the redox/inflammatory profile of the lung is a target of Western diet-induced obesity (WDIO) in a mouse model. To test our hypothesis we compared the redox/inflammatory profile in the lung of mice fed for 16 weeks either a low-fat diet and tap water (LFD) or a high-fat diet (HFD) and 10% fructose in tap water (HFD+F). In relation to LFD mice, the lung of HFD+F MICE showed: reduced antioxidant antioxidant capacity?assessed by bleaching of the ABTS+ (P