RECEPTORS EXPRESSION IN VITAMIN A DEFICIENT RATS

VASQUEZ ME, FERRARI C, CAMPO VERDE FJAHN G, GIMENEZ MS

Estancia Grande-San luis

Congreso; Reunion Anual de la Soc de Biologia de Cyo; 2014

Comision Directiva Soc de Biologia de Cuyo

RECEPTORS EXPRESSION IN VITAMIN A DEFICIENT RATS. Vasquez ME, Ferrari C, Campo Verde FJahn G, Gimenez MS Dpto Bioquímica y Cs biologicas. UNSL. IMIBIO-SL CONICET 5700 San Luis Email: eridnere@gmail.com Vitamin A is an essential nutrient involved in numerous processes. The pleiotropic effects of retinoic acid are mediated by its receptors (RARs). Each family consists of three receptors (α,β,γ). We observed a relationship between retinoic acid and estrogen hormone receptors (ER) and progesterone (RPr). The aim is to determine whether vitamin A deficiency alters the expression of receptors RAR alpha, RE alfa and RPrB in mammary gland. It works with female Wistar rats at weaning in three separate groups. One devour diet sufficient in vitamin A (8 mg retinol as retinyl palmitate/kg diet), one with deficient Vitamin A diet and the third group will receive diet deficient in Vitamin A for seventy- five days to the end of which will be given adequate Vitamin A diet for 15 days. Other 3 experimental groups of 180 days, with diet sufficient in Vitamin A, another diet deficient in Vitamin A and the third group will receive diet deficient in Vitamin A for 150 days after which you will be given diet sufficient in Vitamin A for 30 days. Expression of RAR alpha receptors by RT ?PCR was determined. Expression of RPrB and ER alpha RT-qPCR was determined. Wester ER alpha was conducted. It was observed in 6 month deficient rats decreased expression RPrB about 6 months controls (p< 0.01) , and this reduction in the group refed 6 months (p