Adjustment of a Western Diet-Induced Obesity Mouse Model for Immunometabolic Studies

MARIA CECILIA DELLA VEDOVA1,2, MARCOS D MUÑOZ1,3, MARTIN RINALDI TOSI1, SILVINA GARCIA1, SANDRA E GOMEZ MEJIBA1,3, AND DARIO C RAMIRE

Seattle

Congreso; SFRBM`s; 2014

Sociedad de Radicales Libres

Adjustment of a Western Diet-Induced Obesity Mouse Model for Immunometabolic Studies Maria Cecilia Della-Vedova1,2, Marcos D Muñoz1,3, Martin Rinaldi Tosi1, Silvina Garcia1, Sandra E Gomez Mejiba1,3, And Dario C Ramirez1,2 1Lab. Exp. Med. & Ther., IMIBIO-SL, Nat. Bureau of Sci. & Tech. Res. (CONICET), Argentina, 2Dept. of Biochemstry, School of Chemistry, Biochemistry & Pharmacy-UNSL, Argentina, 3Dept. of Nutrition, School of Health Sciences-UNSL, Argentina The increase in the consumption of chicken fat and fructose, which are cheaper ingredients for foods, coincides with an increase in the prevalence of obesity. Due to the world known difficulties Argentinean scientists to import commercially available diets herein we aimed at developing a mouse model of dietinduced obesity that uses local ingredients and that also highlights the main features of human obesity, including adiposity and insulin resistance (IR). To accomplish our aim we fed male 6 week-old B6 mice for 16 weeks with a rodent chow diet containing either 4 (LFD) or 22% (HFD) p/p chicken fat and water containing or not 10% p/v fructose (F). This experimental design resulted in 4 experimental groups: high fat diet plus water supplemented with 10% fructose (HFD+F); HFD only, low fat diet only (LFD) and LFD+F. The consumption of food and drink as well as body weight gain were measured throughout the experiment. At the end of the feeding period adiposity index, fasten glucemia and IR (glucose tolerance test) were measured. The results (mean values􀁲SEM) showed that compared to mouse from other groups those from the HFD+F group: i) gained more weight (HFD+F:29.41±1.07 vs HFD:27.4􀁲0.93; LFD+F:25.5􀁲0.70; LFD:24.77􀁲0.60); ii) have heavier epididymal fat-pads thus larger adiposity index ([epididymal fat(g)/body weight] x100 (HFD+F:4.38±1.36 vs HFD:3.34±0.27; LFD+F:2.12±0.17; LFD:1.43±0.10); iii) like the LFD+F this group drank more water (ml/mouse/day, HFD+F:8.7±0.46 and LFD+F:7.43±0.47 vs HFD:4.94±0.56 and LFD:4.52±0.70); iv) ate less (mg/mouse/day, HFD+F:2.16±0.07 vs HFD:2.55±0.03; LFD+F:2.35±0.06; LFD:2.95±0.07); v) have more fasten glucemia (HFD+F:271􀁲50 vs HFD:233􀁲50; LFD+F:212±44; LFD:183􀁲45); and vi) are more IR (AUC as arbitrary units, HFD+F:14468􀁲937 vs HFD:12000􀁲450; LFD+F:940􀁲570; LFD:8490􀁲930). A mouse model of diet-induced obesity, which will allow us to compare the contribution of fructose and fat consumption on immunometabolism has been developed and will provide a tissue repository to increase the efficiency of animal usage in projects aimed at dissecting obesity-associated abnormalities. Supported by PROICO-2-3214(FQBYF)/PROICO10-0414(FCS)