PERISPHERAL NERVOUS SYSTEM MAY REGULATE CORPUS LUTEUM LIFE SPAN THROUGH PROGESTERONE RECEPTOR IN CELIAC GANGLIA

GHERSA F; VALLCANERAS S; BURDISSO J; FEUNTES F

San Luis

Congreso; Sociedad de Biología de Cuyo XXXII Reunión Científica Anual; 2014

Stimulating celiac ganglion (CG) with progesterone (Pg), a luteotrophic hormone, ovarian release of Pg is stimulated through superior ovarian nerve (SON). The object of this study is to analyze the effect of Pg, on corpus luteum (CL) of 21 day pregnant rats, through SON by adding Pg to CG using an ex vivo system composed by CG-SON-Ovary, and to investigate through which receptor Pg may be acting in CG. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments. Pg (10-5M) was added in the ganglion compartment [(Pg)g], Controls were not stimulated. Periodic extractions of the ovary incubation liquid were carried out at 30, 60, 120 and 180 min and RIA was performed to quantify Pg release. At 180 min, the luteal mRNA expression of 3β-HSD, 20α-HSD (synthesis and degradation enzymes respectively), Fas and Fas-L were analyzed by RT-PCR. Tissue sections of CG were treated for inmunohistochemistry and incubated with antibodies against Pg receptor (PR) and β III tubulin. ANOVA 1 followed by Tuckey test with a statistical significance of p