DIFFERENT FACTORS PARTICIPATING IN YERSINIA ENTEROCOLITICA BIOFILM FORMATION

GAURÓN, MARÍA CLESTE; CECILIA S. M. LUCERO ESTRADA; DI GENARO SILVIA; TERESA I. CORTIÑAS

Mendoza

Jornada; XXXI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2013

Sociedad de Biología de Cuyo

The enteropathogen Yersinia enterocolitica (Ye) is able to form biofilm at 25°C onto polystyrene but the machinery involved in this phenotype is still unclear. The aim of this work was to elucidate if either the quorum sensing (QS) system or Ye virulence genes are important for biofilm formation. Three wild type Ye strains (two biotype 1A and one 1B), and two mutants (yopP-, yopH-) were used. Biotype 1A strains were challenged against 200, 150 and 100 µg/ml of tannic acid, a QS inhibitor, and evaluated for biofilm formation by the Crystal violet technique. Biotype 1B and mutant strains were only evaluated for biofilm formation. Tannic acid significantly inhibits biofilm formation but the deletion of yopP and yopH significantly increase this phenotype. Several factors are involved during Ye biofilm formation and remained to be investigated; so more investigation is still necessary to completed elucidate the implicated mechanisms.