RAT LUNG EXPOSED TO CADMIUM - EFFECT OF DIFFERENT PROTEIN SOURCES ON THE ANTIOXIDANT DEFENSE SYSTEM

BOLDRINI GABRIEL GIEZI; ALVAREZ SILVINA MONICA; ESCOBAR CORREAS SOPHIE MELANIE; DELLA VEDOVA MARIA CECILIA; PEREZ CHACA MARIA VERONICA; GOMEZ NIDIA NOEMI; GIMENEZ MARIA SOFIA

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2013

Sociedad de Biologia de Cuyo

RAT LUNG EXPOSED TO CADMIUM - EFFECT OF DIFFERENT PROTEIN SOURCES ON THE ANTIOXIDANT DEFENSE SYSTEM Boldrini GG, Álvarez SM, Escobar Correas SM, Della Vedova MC, Perez Chaca MV, Gomez NN, and Giménez MS. Lab de Nutrición y Medio Ambiente, IMIBIO-CONICET. San Luis. Argentina. salvarez@unsl.edu.ar Cadmium (Cd) is a toxic element and an important environmental contaminant. We studied its effect on the antioxidant defense system of rat lung under different diets. 4 lots of female Wistar rats were used: 2 lots received casein (Cas) and 2 lots soybean (Soy) as protein source. Within each group, 1 lot received regular water (control-Co) and the other, 15 ppm of Cd in the drinking water for 60 days. Homogenates were made and TBAR?S and CAT activity were determined. Total RNA was isolated with Trizol and cDNA was obtained. Superoxide Dismutase (SOD), Nuclear factor-erythroid 2 related factor 2 (nrf-2), p47phox, Glutathion peroxidase (GPx) and NADPH Oxidase (NOX-2) were determined by PCR. S28 was the control. TBAR?S showed a significant increase in both Cd groups and CAT activity showed a not significant increase in the Soy groups (higher in the Cd group). Nrf-2 (p