INFLUENCE OF A HIGH FAT DIET ON LIPID METABOLISM OF RAT ADIPOSE TISSUE

SANTILLÁN LD, GIMÉNEZ MS

Congreso; Sociedad de Biologia de Cuyo; 2012

INFLUENCE OF A HIGH FAT DIET ON LIPID METABOLISM OF RAT ADIPOSE TISSUE Santillán LD, Giménez MS Molecular Biochemestry, Fac de Qca, Bioqca y Fcia. UNSL-IMIBIO. E-mail:lucassantillan2011@gmail.com Feeding hypercaloric diet of male Sprague Dawley (SD) rats induces obesity. Our objective was to investigate the effect of a high calorie diet, high-fat (32%), on lipid metabolism of perirenal adipose tissue (TAPR). Triglycerides are preferentially accumulated in TAPR. Two groups of SD rats were used: one fed with high fat diet, and another one fed with control diet, for 12 weeks after weaning. TAPR were quantified total lipids, total cholesterol, free and esterified cholesterol, phospholipids and triglycerides, after separation by TLC. Also, the relative composition of fatty acids (by GC), and the mRNA expression of enzymes and transcription factors involved in lipogenesis and lipolysis (RT-PCR), were determined. High fat diet produced in TAPR an increase of triglycerides and total lipid levels, and fatty acid synthase, glycerol-3-phosphate acyltransferase and SREBP-1c expressions, which are involved in the lipid synthesis, compared with control group. Likewise, mRNA levels of carnitine palmitoyl transferase-CPT1 (regulator of the fatty acids beta oxidation) decreased respect to the control group. Results indicate that administration of a hypercaloric diet- high fat content- in SD rats during 12 weeks stimulates lipogenesis and increases fatty acid oxidation.