Effect of soybean meal on antioxidant enzymes in rats fed high-calorie diet

RAZZETO GS, LUCERO LÓPEZ VR, ESCUDERO NL, GIMÉNEZ MS.

Congreso; Sociedad de Biologia de Cuyo; 2012

Effect of soybean meal on antioxidant enzymes in rats fed high-calorie diet Razzeto GS, Lucero López VR, Escudero NL, Giménez MS. Fac. Qca. Bqca. y Fcia. UNSL. IMIBIO-SL-CONICET. 5700 San Luis. Email: grazzet@unsl.edu.ar High calorie diets induce the generation of reactive oxygen species that cause damage to macromolecules. Soy has antioxidants that play a key role in free radical scavenging. The aim of this study was to evaluate the activity and gene expression through kinetic assays and RT-PCR of: CAT (catalase), SOD (superóxide dismutase), GPx (glutathione peroxidase) y GR (glutathione reductase) in the liver of rats fed high-calorie diets with different protein sources. The animals were divided into two groups: control, fed AIN-93 diet, and experimental, fed AIN-93 diet high-calorie (34.15% sucrose, 42% of calories from fat) for 9 weeks. After that, each group was divided into two: one using casein and the other one using soybean as protein source, respectively. Animals were fed for 45 days and then sacrificed. Thus, the groups being: CC (control casein), CS (control soybean), HC (high-calorie casein) and HS (high-calorie soybean). In the CS group, SOD, GPx, GR and CAT activity were increased in relation to CC (P